Glutatyon S-Transferaz Enziminin Tavuk Karaciğerinden Saflaştırılması ve Karakterizasyonu
نویسندگان
چکیده
In this study, the glutathione S-transferase enzyme (GST; EC 2.5.1.18) was purified with 8.35 EU/mL specific activity, 24.56 times 8% yield, from chicken liver, using ammonium sulfate precipitation and glutathione-agarose affinity chromatography. order to control purity of enzyme, SDS-PAGE performed a single band obtained. The molecular mass subunit calculated as approximately 30.9 kDa. addition, optimum pH value (8.5 in Tris-HCl); ionic strength (150 mM temperature (70 oC); stable Tris-HCl) determined. KM for GSH substrate 0.802 mM, Vmax 1.833 EU/mL; For CDNB, 3.6 2.829 EU/mL.
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ژورنال
عنوان ژورنال: Türk do?a ve fen dergisi
سال: 2023
ISSN: ['2147-303X', '2149-6366']
DOI: https://doi.org/10.46810/tdfd.1242764